2,3,5-Triphenyltetrazolium chloride

2,3,5-Triphenyltetrazolium chloride Basic information
Product Name:2,3,5-Triphenyltetrazolium chloride
Synonyms:2,3,5-TriphenyltetrazoliuM chloride SynonyMs 2,3,5-Triphenyl-2H-tetrazoliuM chloride;TTC,TTZ,Tetrazolium Red;FLJ37239;LGMD2K;10 MG ABTS TABLETS;BCIP P-TOLUIDINE SALT ULTRA PURE GRADE;TSA PENASE CONTACT 55MM TRIPLE-WRAP;RARECHEM AH BS 0067
CAS:298-96-4
MF:C19H15ClN4
MW:334.8
EINECS:206-071-6
Product Categories:substrate;Tetrazolium Salts;Tetrazolium Salts & Formazans;Tetrazolium salt
Mol File:298-96-4.mol
2,3,5-Triphenyltetrazolium chloride Structure
2,3,5-Triphenyltetrazolium chloride Chemical Properties
Melting point 250 °C(lit.)
Boiling point 490.96°C (rough estimate)
density 1.1704 (rough estimate)
refractive index 1.6110 (estimate)
storage temp. 2-8°C
solubility H2O: may be clear to slightly hazy
form powder
color faint yellow
Water Solubility Soluble in water, ethanol and acetone. Insoluble in ether.
λmax247 nm
Sensitive Light Sensitive & Hygroscopic
Merck 14,9744
BRN 3923356
Stability:Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents.
Biological ApplicationsAlgae viability assay; microbial growth assays; transketolase activity screening assays; detecting bacteria,g-hydroxybutyric acid (GHB),microorganisms,myocardial infarction; measuring dehydrogenase activity; treating cancer
InChIKeyPKDBCJSWQUOKDO-UHFFFAOYSA-M
CAS DataBase Reference298-96-4(CAS DataBase Reference)
EPA Substance Registry System2,3,5-Triphenyltetrazolium chloride (298-96-4)
Safety Information
Hazard Codes Xi,Xn
Risk Statements 36/37/38-20/21/22
Safety Statements 26-36
WGK Germany 3
RTECS XF8100000
3-8-10
TSCA Yes
HS Code 29339900
ToxicityLD50 intravenous in mouse: 5600ug/kg
MSDS Information
ProviderLanguage
2,3,5-Triphenyl-2H-tetrazolium chloride English
SigmaAldrich English
ALFA English
2,3,5-Triphenyltetrazolium chloride Usage And Synthesis
Chemical Propertieswhite powder
UsesIn analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between a-ketols and simple aldehydes. For staining plant and animal tissue. Germination indicator in testing the ability of seeds to germinate: G. Lakon, Ber. Dtsch. Bot. Ges. 60, 299, 434 (1942). In histochemical studies. In determination of antibiotics; of dehydrogenases.
Uses2,3,5-Triphenyl-2H-tetrazolium chloride is used as a dye in cell biological studies.
Uses2,3,5-Triphenyltetrazolium chloride is a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia
DefinitionChEBI: An organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion.
General Description2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.
Purification MethodsCrystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.]
2,3,5-Triphenyltetrazolium chloride Preparation Products And Raw materials
Raw materialsSodium hydroxide-->Hydrochloric acid-->Acetic acid-->Sodium nitrite-->Sodium acetate-->Aniline-->Sodium hypochlorite-->Benzaldehyde-->Phenylhydrazine
Triphenyltetrazolium salt Tris(triphenylphosphine)rhodiu Nitroblue Tetrazolium Chloride (NBT) Tetranitroblue tetrazolium chloride 2,3,5-TRIPHENYLTETRAZOLIUM IODIDE 2,3,5-TRIPHENYLTETRAZOLIUM BROMIDE 98+%,2,3,5-TRIPHENYLTETRAZOLIUM BROMIDE 2,3,5-TRIPHENYLTETRAZOLIUM CHLORIDE = TETRAZOLIUM RED,2,3,5-TRIPHENYLTETRAZOLIUM CHLORIDE = TETRAZOLIUM RED 5-Methyl-1H-tertazole 2,3,5-TRIPHENYLTETRAZOLIUM FORMAZAN,1,3,5-TRIPHENYLTETRAZOLIUM FORMAZAN,2,3,4-TRIPHENYLTETRAZOLIUM FORMAZAN Neotetrazolium chloride 1H-Tetrazole-1-acetic acid 3-NITROTETRAZOLIUM BLUE CHLORIDE 50 G TRIPHENYLTETRAZOLIUM CHLORIDEANALYTICAL GRADE Mepiquat chloride ANBT P-ANISYL BLUE TETRAZOLIUM CHLORIDE TPTZ 2-​[[(2-​ethylphenyl)​(2-​hydroxyethyl)​amino]​methyl]​-​3,​3-​difluoro-Propanenitrile Tetrazolium Chloride

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