|  | |  |  | 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Basic information | 
|  |  | 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Chemical Properties | 
 | Melting point | 0.05°C |  | Boiling point | 586.77°C (rough estimate) |  | density | 1.06 g/mL at 20 °C |  | vapor pressure | <0.01 hPa (20 °C) |  | refractive index | n20/D 1.492 |  | Fp | >230 °F |  | storage temp. | Store at +5°C to +30°C. |  | solubility | Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly), Water (Slightly) |  | form | Liquid |  | color | Yellow |  | PH | 5.0-8.0 (10g/l, H2O, 20℃) |  | Water Solubility | Miscible with water. |  | Merck | 13,6793 |  | BRN | 2315025 |  | CAS DataBase Reference | 9036-19-5(CAS DataBase Reference) |  | EPA Substance Registry System | Polyethylene glycol mono(1,1,3,3-tetramethylbutyl)phenyl ether (9036-19-5) | 
|  |  | 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Usage And Synthesis | 
 | Uses | Triton? X-100 has been used: 
 In immunohistochemistry for staining the Flat-mount retinasAlong with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assayTo permeabilise cells during Immunofluorescent microscopic studiesAs a positive control in LDH assay to determine the cell membrane integrityFor estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedureFor the preparation of outer membrane protein exctractAs a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cellsIn the treatment of tissue sections for Immunofluorescence labeling
 |  | Uses | 0·3% Triton? X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis. |  | Uses | Triton?-X has been used: 
 In the permeabilization of cells for immunofluorescence stainingAs a component of lysis buffer in western blot analysisAs a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
 |  | General Description | Octophenolpoly(ethyleneglycolether)n Especially used for purification in membrane research
 Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.
 |  | Biochem/physiol Actions | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. |  | Safety Profile | Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes. |  | Safety Profile | Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits acridsmoke and irritating fumes. | 
|  |  | 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Preparation Products And Raw materials | 
 |