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| | 1,10-Phenanthroline hydrate Basic information |
| | 1,10-Phenanthroline hydrate Chemical Properties |
| Melting point | 100-104 °C(lit.) | | density | 1.10 | | vapor density | 6.2 (vs air) | | storage temp. | Sealed in dry,Room Temperature | | solubility | methanol: soluble200MM (Stock solution is stable for months at –20° C.) | | form | Needle-Like Crystals | | color | Off-white, may become cream-colored during storage | | Water Solubility | <0.01 g/100 mL at 21 ºC | | Merck | 14,7214 | | BRN | 4008096 | | Stability: | Stable. Incompatible with iron, other heavy metals, strong oxidizing agents, strong acids. | | CAS DataBase Reference | 5144-89-8(CAS DataBase Reference) | | EPA Substance Registry System | 1,10-Phenanthroline, monohydrate (5144-89-8) |
| Hazard Codes | T,N | | Risk Statements | 25-50/53 | | Safety Statements | 45-60-61 | | RIDADR | UN 2811 6.1/PG 3 | | WGK Germany | 3 | | RTECS | SF8437000 | | TSCA | Yes | | HazardClass | 6.1 | | PackingGroup | III | | HS Code | 29339990 | | Toxicity | LD50 orally in Rabbit: 132 mg/kg |
| | 1,10-Phenanthroline hydrate Usage And Synthesis |
| Chemical Properties | white crystals or powder | | Uses | When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.
1,10-Phenanthroline monohydrate is an aromatic nitrogen heterocycle that may be used as a chelating agent in the preparation of metal-chelate complexes. | | Uses | 1,10-Phenanthroline monohydrate is useful to study the DNA-protein interactions due to its nuclease activity. It is used as a redox indicator and reagent for spectrophotometric assay of silver. It acts as a chelating ligand for the determination of Fe, Pd and V. It also acts as a matrix metalloproteinase inhibitor. It is a photometric reagent for iron, which forms a red chelate with ferrous (iron II) ion. | | General Description | White crystalline powder or solid. Slight odor. | | Air & Water Reactions | Insoluble in water. | | Reactivity Profile | 1,10-Phenanthroline hydrate is incompatible with strong oxidizers. 1,10-Phenanthroline hydrate is also incompatible with strong acids. 1,10-Phenanthroline hydrate forms complex compounds with ferrous ions. . | | Fire Hazard | Flash point data for 1,10-Phenanthroline hydrate are not available. 1,10-Phenanthroline hydrate is probably combustible. | | Biochem/physiol Actions | Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM. | | Purification Methods | Crystallise its picrate (m 191o) from EtOH; then the free base is liberated with aqueous alkali, dried at 78o/8mm over P2O5 and crystallised from pet ether (b 80-100o). [Cumper et al. J Chem Soc 1188 1962.] It can be purified by zone melting. It has also been crystallised from hexane, *benzene/pet ether (b 40-60o) or sodium-dried *benzene, dried and stored over H2SO4. The monohydrate is obtained by crystallisation from aqueous EtOH or ethyl acetate. It has been crystallised from H2O (300 parts) to give the monohydrate m 102-103o which sublimes at 10-3mm [Fielding & LeFevre J Chem Soc 1811 1951.] The anhydrous compound has m 118o (after drying at high vacuum at 80o) and is also obtained by recrystallisation from pet ether or *C6H6 (70 parts) and drying at 78o/8mm. [UV: Badger et al. J Chem Soc 3199 1951.] IthasapKainH2Oof 4.857 (25o) or 5.02 (20o) and 4.27 in 50% aqueous EtOH (20o). [Albert et al. J Chem Soc 2240 1948]. [Beilstein 23 H 227, 23 II 235, 23/8 V 419.] |
| | 1,10-Phenanthroline hydrate Preparation Products And Raw materials |
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