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| | CI 51180 Basic information |
| Product Name: | CI 51180 | | Synonyms: | 5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1);9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1);Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1);nileblueasulphate;BASIC BLUE 12;BASIC BLUE 12 SULFATE SALT;CI NO 51180;CI 51180 | | CAS: | 3625-57-8 | | MF: | C20H20N3O5S- | | MW: | 414.46 | | EINECS: | 222-832-5 | | Product Categories: | Phenoxazine;marker | | Mol File: | 3625-57-8.mol |  |
| | CI 51180 Chemical Properties |
| Melting point | >300 °C (dec.)(lit.) | | storage temp. | room temp | | solubility | Soluble in water, ethanol | | Colour Index | 51180 | | form | Powder | | color | Dark green to black | | PH Range | 10.1(BLUE)-11.1(RED) | | Water Solubility | Soluble in ethanol, and water (50 g/L) at 25°C. | | λmax | 633 nm | | Biological Applications | Detecting microorganisms; treating virus infectious diseases; photodynamic therapy |
| Hazard Codes | Xi | | Safety Statements | 22-24/25 | | WGK Germany | 3 | | RTECS | DJ1931000 | | HS Code | 3212900000 | | Toxicity | mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952. |
| | CI 51180 Usage And Synthesis |
| Chemical Properties | dark green to black powder | | Uses | Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue
| | Uses | Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites. | | Uses | Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections. | | General Description | Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry. | | Biochem/physiol Actions | Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red. | | Purification Methods | Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.] |
| | CI 51180 Preparation Products And Raw materials |
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