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| | S-Trityl-L-cysteine Basic information |
| | S-Trityl-L-cysteine Chemical Properties |
| Melting point | 182-183 °C (dec.)(lit.) | | Boiling point | 524.7±50.0 °C(Predicted) | | density | 1.1342 (rough estimate) | | refractive index | 111 ° (C=0.8, 0.04mol Ethanolic HCl) | | storage temp. | Sealed in dry,Room Temperature | | solubility | Soluble in DMSO (up to 20 mg/ml). | | form | White to off-white solid. | | pka | 2.22±0.10(Predicted) | | color | White or off-white | | optical activity | [α]25/D +115°, c = 0.8 in 0.04 M ethanolic HCl | | BRN | 2339626 | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 week. | | CAS DataBase Reference | 2799-07-7(CAS DataBase Reference) |
| Hazard Codes | Xn | | Risk Statements | 22 | | Safety Statements | 22-24/25 | | WGK Germany | 3 | | RTECS | AY7710000 | | HS Code | 29309090 | | Toxicity | dog,LDLo,intravenous,150mg/kg (150mg/kg),GASTROINTESTINAL: NAUSEA OR VOMITINGGASTROINTESTINAL: "HYPERMOTILITY, DIARRHEA"BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY),National Technical Information Service. Vol. PB81-109993, |
| | S-Trityl-L-cysteine Usage And Synthesis |
| Description | S-Trityl-cysteine (2799-07-7) is a cell permeable inhibitor of mitotic kinesin Eg5 (IC50 for inhibition of basal ATPase activity = 1 μM; IC50 for inhibition of microtubule-activated ATPase activity = 140 nM). Mean growth concentration (GI50) of 1.31 μM for a panel of 60 different tumor cell lines. | | Chemical Properties | almost white to light yellow granular powder | | Uses | A protected cysteine for peptide synthesis. S-Trityl-L-cysteine (STLC) is a tight-binding inhibitor of Eg5 that prevents mitotic progression. It has proven antitumor activity as shown in the NCI 60 tumor cell line screen. | | Uses | (+)-S-Trityl-L-cysteine, a non-natural, sulfur-containing amino acid is commonly used as a reagent in solution phase peptide synthesis (SPPS). It is also used as a metal-binding agent to synthesize substituted ferrocenoyl peptide conjugates using HBTU peptide coupling reagent for the cation-sensing applications solution via peptide-metal interactions. | | Definition | ChEBI: (2R)-2-amino-3-[(triphenylmethyl)thio]propanoic acid is a benzenoid aromatic compound. | | Biological Activity | Potent, cell-permeable, selective inhibitor of mitotic kinesin Eg5, a protein required for establishing and maintaining a bipolar spindle. Inhibits basal ATPase activity (IC 50 = 1 mM) and microtubule-activated ATPase activity of Eg5 (IC 50 = 140 nM). Induces mitotic arrest in HeLa cells with an IC 50 of 700 nM. Displays antitumor activity. | | storage | Store at +4°C | | References | 1) DeBonis et al. (2004) In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumour activities; Mol. Cancer Ther. 3 1079
2) Brier et al. (2004) Identification of the protein binding region of S-trityl-L-cysteine, a new potent inhibitor of mitotic kinesin Eg5; Biochemistry 43 13072
3) Skoufias et al. (2006) S-trityl-l-cysteine is a reversible, tight-binding inhibitor of the human kinesin eg5 that specifically blocks mitotic progression; J. Biol. Chem., 281 17559 |
| | S-Trityl-L-cysteine Preparation Products And Raw materials |
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