Lysozyme

Lysozyme Basic information
Description References
Product Name:Lysozyme
Synonyms:LYSOZYME 3x crystallised extrapure;Lysozyme Agarose from chicken egg white;Mucopeptide N-acetylmuramoylhydrolase, Muramidase;LYSOZYME FROM CHICKEN EGGWHITE CA. 150 000 U/MG CRYST.;Lysozyme from chicken egg white,Mucopeptide N-acetylmuramoylhydrolase, Muramidase;Lysozyme [from chicken egg, 50000 units/mg];Lysozymes;REDUCED LYSOZYME, WATER SOLUBLE
CAS:12650-88-3
MF:C125H196N40O36S2
MW:2899.27014
EINECS:235-747-3
Product Categories:enzyme;Enzymes
Mol File:12650-88-3.mol
Lysozyme Structure
Lysozyme Chemical Properties
vapor pressure 0Pa at 25℃
storage temp. -20°C
form powder
color white
PHpH(15g/l, 25℃) : 3.0~5.0
Water Solubility Soluble in water at 10mg/ml.
Safety Information
Hazard Codes B
Safety Statements 23-24/25-22
WGK Germany 3
RTECS OL5989850
3-10
HS Code 35079090
Toxicitymouse,LD50,intraperitoneal,5800mg/kg (5800mg/kg),BEHAVIORAL: CHANGES IN MOTOR ACTIVITY (SPECIFIC ASSAY)LUNGS, THORAX, OR RESPIRATION: RESPIRATORY DEPRESSIONBEHAVIORAL: ATAXIA,Oyo Yakuri. Pharmacometrics. Vol. 21, Pg. 587, 1981.
MSDS Information
ProviderLanguage
Lysozyme English
SigmaAldrich English
Lysozyme Usage And Synthesis
DescriptionLysozyme belongs to a group of enzymes that are known as glycoside hydrolases. It is found in animal tissues, organs, serum as well as in tears, nasal secretions, cervical mucus, and the white of avian eggs. Lysozyme breaks the carbonhydrate chains of the bacterial skin, destroying the structural integrity of the cell wall. Thereby, it has antibacterial properties and was one of the first antibiotics studied by Sir Fleming, the discover of penicillin.
Lysozyme is used either alone or in combination with other synergic compounds as an excellent preservative against many food spoiling microorganisms for fruits & vegetables, tofu & bean curd, seafood & meats, wines & sakes. Organic wines use it to reduce sulfites. It is used in non-pasteurized beer. It has been added to baby formula (to aid digestibility) and gastrointestinal treatments for the elderly.  Lysozyme is used in skin care, to cure and prevent acne and bed sores, as well as in optical, dental, and oral conditions.
References[1] http://ase.tufts.edu/biology/MolecVisual/bio152/rightlyso.html#top
[2] http://www.xtend-life.com/information/ingredients/lysozyme
[3] http://www.bioseutica.com/products/lysozyme

Chemical PropertiesLysozyme (muramidase) hydrolyzes preferentially the beta-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A somewhat more limited activity is exhibited towards chitin oligomers. Lysozyme is of widespread distribution in animals and plants. That which has been most extensively studied is from hen egg white (lysozyme C). Lysozyme is also found in mammalian secretions and tissues, saliva, tears, milk, cervical mucus, leukocytes, kidneys, etc. Lysozyme is inhibited by surface-active reagents such as dodecyl sulfate, alcohols and fatty acids. Imidazole and indole derivatives are inhibitors via formation of change-transfer complexes. Useful for the rapid isolation of RNA from gram negative bacteria. Can be used to prepare small amounts of plasmid DNA.
UsesCatalyzes the hydrolysis of peptidoglycans found in bacterial cell walls. Lysozyme catalyzes is utilized for the hydrolysis of peptidoglycans found in bacterial cell walls. It is used as a precursor in the production of spheroplasts. It is also used as a preservative against many food spoiling microorganisms. It is used in skin care in order to cure and prevent acne and bed sores, dental and oral conditions. It is used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. Further, it is used in non-pasteurized beer.
UsesLysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.
ApplicationLysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells.It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
General DescriptionLysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).
Biochem/physiol ActionsLysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme Preparation Products And Raw materials
Raw materialsDOWEX MAC-3 ION EXCHANGE RESIN-->OVALBUMIN
Preparation ProductsLYSOZYME-->Xanthan gum-->cecropin
Aluminum acetylacetonate Lysozyme hydrochloride hen egg lysozyme TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) N-BUTYLISOCYANIDE PHENYLSELENOL Tris(2,4-pentanedionato)chroMiuM(III) SALCOMINE 2,4-PENTANEDIONE, SILVER DERIVATIVE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Tosylmethyl isocyanide Lysozyme Cupric acetylacetonate Ethyl isocyanoacetate TERT-BUTYL ISOCYANIDE Ferric acetylacetonate COBALT ETHYLENE DIAMINE CHLORIDE Benzyl isocyanide Lysozymes

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