THIOSTREPTON

THIOSTREPTON Basic information
Product Name:THIOSTREPTON
Synonyms:Thiostrepton (200 mg);BryaMycin, Thiactin, AlaninaMide, X 146, A 8506, 6761-31;Thiostrepton, froM StreptoMyces sp.;x146;THIOSTREPTON;Alaninamide, L-threonyl-(4S)-2-(1Z)-1-amino-1-propenyl-4,5-dihydro-4-thiazolecarbonyl-2-(1S,2S,3R)-1-amino-2,3-dihydroxy-2-methylbutyl-4-thiazolecarbonyl-2-(5R,6S)-6-2-(1S,2R)-1-amino-2-hydroxypropyl-4-thiazolyl-5-N-(7R,8S)-2-carboxy-7,8-dihydro-8-hydroxy;THIOSTREPTION;thiostrepton from streptomyces azureus
CAS:1393-48-2
MF:C72H85N19O18S5
MW:1664.89
EINECS:215-734-9
Product Categories:Inhibitors;BETAPACE;Peptide Synthesis/Antibiotics;Amino Acids & Derivatives;Intermediates & Fine Chemicals;antibiotic;Antibacterial;Antibiotics;Antibiotics A to;Antibiotics by Application;Antibiotics T-ZAntibiotics;Chemical Structure Class;Gene Regulation and Expression;Pharmaceuticals;Organics;RNA-Protein Translation InhibitorsMore...Close...;Genetic Marker SelectionAntibiotics;Interferes with Protein SynthesisSpectrum of Activity;L - ZAntibiotics;Mechanism of Action;PeptidesCell Signaling and Neuroscience
Mol File:1393-48-2.mol
THIOSTREPTON Structure
THIOSTREPTON Chemical Properties
Melting point 248-257°C (dec.)
alpha D23 -98.5° (glacial acetic acid); -61° (dioxane); -20° (pyridine)
density 1.0824 (rough estimate)
refractive index 1.6700 (estimate)
storage temp. Sealed in dry,Store in freezer, under -20°C
solubility Soluble in DMSO or chloroform
form Off-white powder
pka10.43±0.46(Predicted)
color Off-white
Water Solubility 0.24g/L(28 ºC)
Merck 13,9440
Stability:Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
Safety Information
Hazard Codes Xn
Risk Statements 22
Safety Statements 22-24/25
WGK Germany 3
RTECS XN6300100
10-21
HS Code 29419090
ToxicityLD50 oral in mouse: > 1gm/kg
MSDS Information
ProviderLanguage
SigmaAldrich English
THIOSTREPTON Usage And Synthesis
DescriptionThe mammalian transcription factor forkhead box M1 (FoxM1) is induced during G1 phase, with expression continuing through S phase and mitosis. Thiostrepton is a natural peptide thiazole antibiotic that inhibits FoxM1 in mammalian cells, preventing the expression of FoxM1-regulated genes, which includes FoxM1 itself. Through this mechanism, thiostrepton prevents proliferation and induces apoptosis in human cancer cells. These effects correlate with the ability of thiostrepton to act as a proteasome inhibitor.
Chemical PropertiesOff-White Solid
Usesbeta-adrenergic agonist
UsesThiostrepton is a macrocyclic antibiotic incorporating thiazoles and other atypical amino acids. Patented in 1961, thiostrepton has been used as an antibiotic and acts by binding to ribosomes to prevent the binding of the EF-G elongation factor and GTP to the 50S ribosomal subunit. Thiostrepton is an inducer of tipA, a gene that controls the bacterial transcription regulators, TipAL and TipAS, that are central regulators in multidrug resistance. Thiostrepton is closely related to siomycin, a recently discovered inhibitor of oncogenic transcription factor, FoxM1.
UsesA protein synthesis-inhibiting antibiotic
UsesNatural antibiotic derived from Streptomyces.
DefinitionChEBI: A heterodetic cyclic peptide, in which the cyclisation step involves a formal lactonisation between the carboxy group of a quinaldic acid-based residue and a secondary alcohol. An antibiotic that inhibits bacterial protein synthesis. Also acts as an antitu or agent.
General DescriptionA thiazole-containing peptide antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit. Inhibits the function of elongation factor G (EF-G) and the dissociation of EF-G from the ribosome. The thiostrepton-resistant gene is also commonly used as a selective marker for recombinant DNA/plasmid technologies.
Biochem/physiol ActionsPrimary TargetEF-G
in vitrothiostrepton inhibits the transcriptional activity and foxm1 expression, and induces strong apoptosis in human cancer cells of different origin that correlates with suppression of foxm1, including leukemia, neuroblastoma, liver cancer, melanoma and prostate cancer cells. thiostrepton binds foxm1 on the promoter site to inhibit transcriptional activity of foxm1 through the foxm1 autoregulation mechanism [1].
in vivothiostrepton suppressed tumor growth in a human breast cancer xenograft model. treatment with developed micelle-thiostrepton nanoparticles decreased xenograft tumor growth induced by the human mda-mb-231 breast and hepg2 liver cancer cell lines. these apoptosis activities in drug-treated tumors were correlated with in vivo suppression of oncogenic foxm1 [1].
References1) Bowen et al. (2005), Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain; J. Biol. Chem., 280 2934 2) Gonzalez et al. (2007), Thiostrepton inhibition of tRNA delivery to the ribosome; RNA, 13 2091 3) Kwok et al. (2008), Thiostrepton selectively targets breast cancer cells through inhibition of forkhead box M1 expression; Mol. Cancer Ther., 7 2022
THIOSTREPTON Preparation Products And Raw materials
N,N'-Diisopropylethylenediamine (2,6-DIMETHYLPYRIDIN-4-YL)METHANOL CHEMPACIFIC 38149 2-Pyridinecarboxylicacid,4,6-dimethyl-(9CI) METHYL-THIAZOL-4-YLMETHYL-AMINE METHYL-(2-METHYL-THIAZOL-4-YLMETHYL)-AMINE 2-Pyridinecarboxylicacid,5,6-dimethyl-(9CI) (4-METHYL-1,3-THIAZOL-2-YL)METHYLAMINE THIOSTREPTON 2-HYDROXY-2-PYRIDYL ETHYLAMINE N-METHYL-1-(3-METHYLPYRIDIN-2-YL)PROPAN-2-AMINE 1-PYRIDIN-2-YL-ETHANOL 3-METHYL-4-ETHYL PYRIDINE 2-FORMYL-5-PICOLINE 6-HYDROXYMETHYL-PYRIDINE-2-CARBOXYLIC ACID ETHYL ESTER 4-ETHYL-2-METHYLPYRIDINE (6-ethylpyridin-2-yl)methanol (2-Thiazolyl)methylamine

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