Deoxyribonuclease

Deoxyribonuclease Basic information
Product Name:Deoxyribonuclease
Synonyms:Deoxyribonuclease I from Bovine Pancreas(Filtered);CHRYSENE NEAT;DeoxyribonueleaseⅠ (Dnase I);BENZO(A)PYRENE 50UG/ML IN TOLUENE;Deoxyribonuclease I from Bovine Pancreas(Ribonuclease&Protease Free);Deoxyribonuclease I from bovine pancreas(Recombinant);DEOXYRIBONUCLEASE I FROM BOVINEPANCREAS STANDARDIZ;DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, LYOPH. ~3000 U/MG
CAS:9003-98-9
MF:NULL
MW:0
EINECS:232-667-0
Product Categories:enzyme;Modifying EnzymesApplication Index;Protein and Nucleic Acid IsolationEnzyme Class Index;3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Elisa Kit-plant ELISA Kit
Mol File:Mol File
Deoxyribonuclease Structure
Deoxyribonuclease Chemical Properties
density 100 g/mL at 20 °C
vapor pressure 0.004Pa at 25℃
storage temp. -20°C
solubility Soluble in 0.15 M sodium chloride (5.0mg/L) clear.
form solution (clear, colorless)
color white
Water Solubility 125g/L at 25℃
Merck 13,2923
LogP-1.3 at 20℃
EPA Substance Registry SystemNuclease, deoxyribo- (9003-98-9)
Safety Information
Safety Statements 23-24/25-22
WGK Germany 3
RTECS RF0750000
10-21
TSCA Yes
HS Code 35079090
MSDS Information
ProviderLanguage
SigmaAldrich English
Deoxyribonuclease Usage And Synthesis
UsesDeoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis
UsesDeoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
UsesAmplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.
General DescriptionDNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
  • Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
  • Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
  • DNase I structure resembles the structure of to exonuclease III. It includes two central ? sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]
Flammability and ExplosibilityNotclassified
Biochem/physiol ActionsDNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Clinical UseDNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.
Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.
Deoxyribonuclease Preparation Products And Raw materials
DNA, SINGLE STRANDED, IMM. ON CELLULOSE, FROM CALF THYMUS* METHYL ISOCYANOACETATE Ursodeoxycholic acid Phosphatase, Acid froM Wheat GerM DnaseⅠ PANCREATICCANCER Deoxyribonuclease Ribonuclease A Chenodeoxycholic acid (-)-2-[METHYLAMINO]-1-PHENYLPROPANE Recombinant RNasin Ribonucleic acid DEOXYRIBONUCLEIC ACID SODIUM SALT EC 3.1.31.1 Nuclease, streptococcal deoxyribo- MUNG BEAN NUCLEASE DEOXYRIBONUCLEIC ACID Alkaline Phosphatase

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