Methylene Blue

Methylene Blue Basic information
Product Name:Methylene Blue
Synonyms:Phenothiazin-5-iuM,3,7-bis(diMethylaMino)-, chloride (1:1);Loeffler’s alkaline methylene blue stain solution;Methylene Blue – potassium hydroxide solution;Methylene Blue, HARLECO?;Methylene Blue, An ethidium bromide alternative for the visualization of nucleic acids in gels;SANDOCRYL BLUE B-RL;TETRAMETHYLTHIONINE CHLORIDE;S NO 1038
CAS:61-73-4
MF:C16H18ClN3S
MW:319.85
EINECS:200-515-2
Product Categories:Analytical Chemistry;Ion Association;Phenothiazine;Indazoles;Analytical reagent
Mol File:61-73-4.mol
Methylene Blue Structure
Methylene Blue Chemical Properties
Melting point 190 °C (dec.)(lit.)
density 1.0 g/mL at 20 °C
refractive index n20/D1.347
Fp 45 °C
storage temp. room temp
solubility Soluble in water, ethanol, ethylene glycol, methyl cellosolve
Colour Index 52015
form Liquid
pka2.6, 11.2(at 25℃)
color Green
Specific Gravity0.98
OdorOdorless
Water Solubility 40 g/L (20 ºC)
λmax661 nm
Merck 14,6060
BRN 3641570
Biological ApplicationsDetecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases
NIST Chemistry ReferenceMethylene blue(61-73-4)
EPA Substance Registry SystemMethylene blue (61-73-4)
Safety Information
Hazard Codes Xn
Risk Statements 10-41-22-20/21/22-36/37/38
Safety Statements 26-39-36
RIDADR UN 1993 3/PG 3
WGK Germany 3
RTECS SP5740000
HS Code 32049010
Hazardous Substances Data61-73-4(Hazardous Substances Data)
ToxicityLD50 oral in rat: 1180mg/kg
MSDS Information
ProviderLanguage
Methylene Blue English
SigmaAldrich English
ACROS English
ALFA English
Methylene Blue Usage And Synthesis
Chemical PropertiesMethylene blue is a heterocyclic aromatic chemical compound with the molecular formula C16H18N3SCl. It has many uses in a range of different fields, such as biology and chemistry. At room temperature it appears as a solid, odorless, dark green powder, that yields a blue solution when dissolved in water. The hydrated form has 3 molecules of water per molecule of methylene blue. Methylene blue should not be confused with methyl blue, another histology stain, new methylene blue, nor with the methyl violets often used as pH indicators.
UsesMethylene Blue, 1% w/v aqueous solution is used as a dye in different staining procedures viz. Wright's stain and Jenner's stain. It also serves as an indicator and medicine. Further, it is used to examine RNA and DNA under the microscope or in a gel. It is widely used as a redox indicator in analytical chemistry. It is also used in the sulfide analysis.
UsesA useful inhibitor of cGMP-mediated processes and a useful stain
DefinitionChEBI: An organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties.
General DescriptionMethylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
HazardToxic by ingestion.
Biological ActivityBiological stain and redox indicator. Inhibits tau filament formation (IC 50 = 1.9 μ M). Also inhibits soluble guanylyl cyclase.
Biochem/physiol ActionsMethylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm. Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans
Clinical UseMethylene blue is 3,7-bis(dimethylamino)-phenazathioniumchloride (Urised). The compound occurs as a dark greencrystalline powder with a metallic appearance that is solublein water (1:25) and alcohol (1:65).
Methylene blue has weak antiseptic properties that makeit useful for the treatment of cystitis and urethritis. The actionof methylene blue is considered to be bacteriostatic.The compound colors the urine and stool blue green.
Safety ProfilePoison by ingestion,intraperitoneal, intravenous, and subcutaneous routes.Human systemic effects: cyanosis, blood changes.Experimental reproductive effects. Mutation datareported. When heated to decomposition it emits verytoxic fumes of NOx, SOx, and
Veterinary Drugs and TreatmentsMethylene blue is used primarily for treating methemoglobinemia secondary to oxidative agents (nitrates, chlorates) in ruminants. It is also employed occasionally as adjunctive or alternative therapy for cyanide toxicity.
Intra-operative methylene blue is also being used to preferentially stain islet-cell tumors of the pancreas in dogs in order to aid in their surgical removal or in determining the animal’s prognosis.
Purification MethodsCrystallise the chloride from 0.1M 664 2HCl (16mL/g), the crystals are separated by centrifugation, washed with chilled EtOH and diethyl ether, and dried under vacuum. Crystallise it from 50% aqueous EtOH, wash it with absolute EtOH, and dry it at 50-55o for 24hours. It has also been crystallised from *benzene/MeOH (3:1). It has been salted out with NaCl from a commercial concentrated aqueous solution, then crystallised from water, and dried at 100o in an oven for 8-10hours. [Beilstein 27 III/IV 5152.]
Methylene Blue Preparation Products And Raw materials
Raw materialsN,N-Dimethylaniline-->Sodium dichromate dihydrate-->N-Methylaniline-->N,N-Dimethyl-1,4-phenylenediamine-->Chromic acid-->thiazine-->Aluminium sulfate hydrate
Preparation ProductsBasic Green 5
NEW METHYLENE BLUE N, ZINC CHLORIDE DOUB LE SALT (C.I. 52030) METHYLENE BLUE ZINC CHLORIDE DOUBLE SA&,METHYLENE BLUE ZINC CHLORIDE DOUBLE SALT FOR MICROSCOPY,CI 52015 CI-52015 EOSIN METHYLENE BLUE, ACCORDING TO MAY-GRüNWALD 100 G METHYLENE BLUE ACID BLUE 119 MAY-GRüNWALD'S EOSINE-METHYLENE BLUE SOLUTION MODIFIED FOR MICROSCOPY EhrlichReagent EOSIN METHYLENE BLUE FOR MICROSCOPY BLOCKING REAGENT GE WRIGHTS DIP STAT POLYCHROME METHYLENE BLUE STAIN METHYLENE BLUE BUFFERED EOSIN METHYLENE BLUE, SOLUTION ACCORDING TO MAY-GRüNWALD CI 52015 1,9-DIMETHYL-METHYLENE BLUE LITHIUM PERIODATE METHYLENE BLUE DOUBLE SALT OF ZINC OF THE METHYL THIONINE CHLORIDE EOSIN METHYLENE-BLUE FOR MICROSCOPY

Email:[email protected] [email protected]
Copyright © 2024 Mywellwork.com All rights reserved.