2-Nitrophenyl-beta-D-galactopyranoside

2-Nitrophenyl-beta-D-galactopyranoside Basic information

Product Name:	2-Nitrophenyl-beta-D-galactopyranoside
Synonyms:	2-Nitrophenyl hexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;beta-D-Galactopyranoside, 2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside, o-nitrophenyl, beta-D-;o-Nitrophenyl beta-galactoside;2-Nitrophenyl-β-D-galactopyranoside;ONPG o-Nitrophenyl-?-D-galactoside
CAS:	369-07-3
MF:	C12H15NO8
MW:	301.25
EINECS:	206-716-1
Product Categories:	Enzyme substrates;Carbohydrates & Derivatives;Substrates;Biochemistry;Galactose;Glycosides;Sugars;substrate
Mol File:	369-07-3.mol

2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties

Melting point	186-193 °C (dec.)
Boiling point	442.39°C (rough estimate)
alpha	-70 º (c=1, H2O)
density	1.3709 (rough estimate)
refractive index	-68.8 ° (C=1, H2O)
storage temp.	2-8°C
solubility	DMSO (Slightly), Water (Slightly, Heated, Sonicated)
pka	12.69±0.70(Predicted)
form	Powder
color	White to very slightly yellow
optical activity	[α]20/D 70±1°, c = 1% in H2O
Water Solubility	Soluble in water.
BRN	92207
InChIKey	KUWPCJHYPSUOFW-RMPHRYRLSA-N
CAS DataBase Reference	369-07-3(CAS DataBase Reference)
NIST Chemistry Reference	Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)

Safety Information

MSDS Information

Provider	Language
2-Nitrophenyl-beta-D-galactopyranoside	English
SigmaAldrich	English
ACROS	English
ALFA	English

2-Nitrophenyl-beta-D-galactopyranoside Usage And Synthesis

Chemical Properties	White Crystalline Solid
Uses	2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
Definition	ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
General Description	ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
Biochem/physiol Actions	β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Purification Methods	Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]

2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials

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