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| | Bromocresol Purple Basic information |
| Product Name: | Bromocresol Purple | | Synonyms: | 4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-methylphenol)s,s-dioxide;4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-o-cresol)s,s-dioxide;4,4’-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-b-methylphend]S,S-dioxide;Bromocresol purple indicator solution (for microbial limit check);3',3''-DIBROMO-O-CRESOLSULFONPHTHALEIN;Bromocresol Purple, indicator grade, pure;Phenol, 4,4-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis2-bromo-6-methyl-;BCP | | CAS: | 115-40-2 | | MF: | C21H16Br2O5S | | MW: | 540.22 | | EINECS: | 204-087-8 | | Product Categories: | Sulfonephthalein;Organics;Analytical Chemistry;Indicator (pH);pH Indicators;Stains and Dyes | | Mol File: | 115-40-2.mol |  |
| | Bromocresol Purple Chemical Properties |
| Melting point | 240 °C (dec.) (lit.) | | density | 1.6509 (estimate) | | Fp | 36 °C | | storage temp. | Store at +5°C to +30°C. | | solubility | ethanol: soluble1mg/mL | | pka | 6.21, 6.3, 6.4(at 25℃) | | form | Solid | | Boiling point | 590.2±50.0 °C(Predicted) | | color | Purplish-brown | | PH | pH : 5.2～6.8 | | PH Range | 5.2(yellow)-6.8(purple) | | Water Solubility | practically insoluble | | λmax | 419nm, 433nm, 591nm | | Merck | 14,1387 | | BRN | 359618 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | Major Application | Waveguides, fuel cells, sensors, sol-gel technology, display device, falsification-proof security paper, paints, detergents, identifying fresh and stale rice, detecting lactic acid bacteria, dental materials, vaginal infection test method | | InChIKey | ABIUHPWEYMSGSR-UHFFFAOYSA-N | | CAS DataBase Reference | 115-40-2(CAS DataBase Reference) | | EPA Substance Registry System | Bromcresol Purple (115-40-2) |
| | Bromocresol Purple Usage And Synthesis |
| Chemical Properties | Crystalline | | Uses | As indicator, pH 5.2 yellow; pH 6.8 purple. | | Uses | It is used as a pH indicator and to measure serum albumin concentrations. It is used as a pH indicator (5.2-6.8 : yellow to blue-purple) and also used in spectrophotometric determination of serum-albumin. | | Uses | Bromocresol purple has been used as a pH indicator in the yeast culture media. It has been used as a pH indicator in the culture media of Geobacillus stearothermophilus subsp. Calidolactis. | | Definition | ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure
serum albumin concentrations. | | General Description | Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts. | | Biochem/physiol Actions | Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts. | | Purification Methods | Dissolve the dye in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase at 419nm. It can also be recrystallised from *benzene. It is an indicator: at pH 5.2 (yellow) and pH 6.8 (purple). [Beilstein 19/3 V 460.] |
| | Bromocresol Purple Preparation Products And Raw materials |
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