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| | 4-Methylumbelliferyl beta-D-galactoside Basic information |
| | 4-Methylumbelliferyl beta-D-galactoside Chemical Properties |
| Melting point | 258-263 °C (dec.) | | alpha | -64 º (c=1, pyridine, after) | | Boiling point | 626.9±55.0 °C(Predicted) | | density | 1.522±0.06 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in DMSO (40 mg/ml) | | form | Powder | | pka | 12.73±0.70(Predicted) | | color | White to slightly off-white | | Water Solubility | soluble | | Sensitive | Moisture Sensitive & Hygroscopic | | BRN | 94673 | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month. | | CAS DataBase Reference | 6160-78-7(CAS DataBase Reference) |
| | 4-Methylumbelliferyl beta-D-galactoside Usage And Synthesis |
| Description | 4-MUG (6160-78-7) is a versatile fluorogenic substrate for ?-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3? This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm | | Chemical Properties | White Powder | | Uses | Substrate for fluorometric assay of ?-D-galactosidase | | Uses | It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium. | | Definition | ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position. | | References | Chiu et al. (2017), Measuring ?-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1
Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042
Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ?-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067 |
| | 4-Methylumbelliferyl beta-D-galactoside Preparation Products And Raw materials |
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