Dehydrocholic acid

Dehydrocholic acid Basic information
Description References
Product Name:Dehydrocholic acid
Synonyms:7,12-trioxo-(5-beta)-cholan-24-oicaci;7,12-trioxo-(5beta)-cholan-24-oicaci;Acide dehydrocholique;acidedehydrocholique;Bilidren;Bilostat;Biochol;Cholagon
CAS:81-23-2
MF:C24H34O5
MW:402.53
EINECS:201-335-7
Product Categories:API;APURONE;Inhibitors;Bile Acids;Biochemistry;Steroids;81-23-2
Mol File:81-23-2.mol
Dehydrocholic acid Structure
Dehydrocholic acid Chemical Properties
Melting point 238-240 °C
alpha 29 º (c=2, dioxane)
Boiling point 444.65°C (rough estimate)
density 1.0687 (rough estimate)
vapor pressure 0.013Pa at 20℃
refractive index 30.5 ° (C=2, Dioxane)
storage temp. Sealed in dry,Room Temperature
solubility ethanol: 10 mg/mL
form Solid
pkapKa 5.12(H2O t = 20 c > CMC) (Uncertain)
color White to Off-White
Water Solubility 65mg/L(30 ºC)
Merck 14,2868
BRN 3226734
LogP1.64 at 22℃ and pH5.7
Surface tension56.9mN/m at 63mg/L and 20℃
CAS DataBase Reference81-23-2(CAS DataBase Reference)
NIST Chemistry ReferenceDehydrocholic acid(81-23-2)
EPA Substance Registry SystemCholan-24-oic acid, 3,7,12-trioxo-, (5.beta.)- (81-23-2)
Safety Information
Safety Statements 24/25
WGK Germany 2
RTECS FZ2300000
TSCA Yes
HS Code 2918300000
MSDS Information
ProviderLanguage
3,7,12-Trioxo-5beta-cholan-24-oic acid English
SigmaAldrich English
ALFA English
Dehydrocholic acid Usage And Synthesis
DescriptionDehydrocholic acid is a semisynthetic bile acid, which is made by the oxidation of cholic acid by chromic acid. It can increase the output of bile by the liver and the filling of the gallbladder. Dehydrocholic acid aids the digestion of fats and increases absorption of fat soluble vitamins.
It is used as a gastrointestinal agent that stimulates the flow of bile into the duodenum (cholagogue) or stimulate the production of bile by the liver (choleretic). It is also used as laxative to relief constipation, diuretic, and a diagnostic aid.
References[1] Giancarlo Cravotto, Arianna Binello, Luisa Boffa, Ornelio Rosati, Marco Boccalini, Stefano Chimichi (2006) Regio- and stereoselective reductions of dehydrocholic acid, Steroids, 469-475
[2] https://pubchem.ncbi.nlm.nih.gov/compound/dehydrocholic_acid
Chemical Propertieswhite to off-white amorphous powder
OriginatorDehydrocholic Acid,New Zealand Pharmaceuticals Limited (NZP)
Usesantibacterial
UsesDehydrocholic Acid is a derivative of Cholic Acid (C432600). a choleretic produced by, and isolated from liver cells.
DefinitionChEBI: 3,7,12-trioxo-5beta-cholanic acid is an oxo-5beta-cholanic acid in which three oxo substituents are located at positions 3, 7 and 12 on the cholanic acid skeleton. It has a role as a gastrointestinal drug. It is an oxo-5beta-cholanic acid, a 7-oxo steroid, a 12-oxo steroid and a 3-oxo-5beta-steroid. It is a conjugate acid of a 3,7,12-trioxo-5beta-cholan-24-oate.
Manufacturing ProcessA.) Oxidation of cholic acid:
A solution, consisting of 15.40 g of cholic acid and 18.75 g of anhydrous sodium acetate in a solvent mixture of 20 ml of ethyl acetate, 30 ml of glacial acetic acid, and 30 ml of water, was prepared. This solution was cooled to 20°C. Chlorine gas was bubbled into the solution with vigorous stirring while the reaction temperature was maintained at 20°C. The chlorine was delivered at a constant rate of about 2.5 g per hour over a 4-hour period. The total amount of chlorine gas was 9.80 g which corresponds to about 3.68 moles per mole of cholic acid, or approximately a 23% excess. The solution temperature was maintained in the range of 16° to 20°C during the entire addition of chlorine. Initially the cholic acid solution was very dark-colored. As the reaction progressed, the solution became pale yellow and a precipitate of sodium chloride deposited. A considerable amount of product and sodium chloride precipitated during the latter stages of the reaction so that the final reaction mixture was a heavy slurry which was difficult to stir. After the addition of chlorine was complete, the slurry was aged one hour with stirring at 20°C. The excess chlorine was then discharged by dropwise addition of 10% aqueous sodium sulfite until the solution gave a negative test to starchiodide paper. The semi-crystalline slurry was then diluted with water to raise the total volume to 225 ml. The water was added dropwise with stirring over a 1-hour period. The ethyl acetate was then distilled off at 65-88°C. The resulting crystalline slurry was cooled to below 70°C and filtered through a sintered-glass funnel of medium porosity. The filter cake was washed until the filtrate gave a negative halide test with silver nitrate solution and then was sucked partially dry on the funnel. Drying was completed in a drier at 110°C for 3 hours. The product was crude pale tan dehydrocholic acid. Yield 14.3 (95%); M.P. 225-231°C.
B.) Purification of dehydrocholic acid:
To a chromatographic column, packed with 6.67 g of charcoal ("Nuchar C") with layers of sea sand at either end, 75 ml of acetone was added to wet the carbon. The column was heated to 40°C, and 25 ml of acetone was drained off. A solution of 20 g of dry crude dehydrocholic acid in 500 ml of acetone was poured into a reservoir atop the column and maintained in this reservoir at 40°C. This solution was then allowed to drop through the column at a constant rate over a 3-hour period. The column was then washed with 250 ml of acetone flowing through the column at a constant rate over a 1-hour-period at 40°C. The column effluent and wash acetone were combined and concentrated to a residual volume of about 100 ml which resulted in the formation of a thick slurry. The slurry was cooled with stirring at 0° to 5°C and aged for 30 min at this temperature. The slurry was filtered and the filter cake washed with cold acetone. The filter cake of U.S.P. dehydrocholic acid was sucked partially dry on the filter and then dried at 110°C for 3 hours. Yield 15 g to 17 g (75% to 85%).
A second crop of crystals was obtained from the combined filtrate and wash liquid from the first crop filtration. This mixture, which initially had a volume of about 100 ml, was concentrated to 20 ml. 10 ml of water was added to the solution and 10 ml of acetone mixed with a small amount of water distilled off. The residual thick slurry of dehydrocholic acid was cooled to 0-5°C, aged at this temperature with stirring for 30 min, and filtered. The filter cake was washed with acetone at 0°C, partially dried by suction on the filter, and then dried for three hours at 110°C. Yield 1 to 2 g (5% to 10%).



Brand nameDecholin Sodium (Bayer).
Therapeutic FunctionCholeretic, Diuretic, Diagnostic aid
Biochem/physiol ActionsDehydrocholic acid is an oxidation product of cholic acid by chromic acid that is not present in physiological conditions. When used in animal experiments, it stimulates bile secretion.
Herbac 3-Oxo-5beta-cholanoic Acid 4-OXO-CYCLOHEXANECARBOXALDEHYDE Ethyl 2-(Chlorosulfonyl)acetate Ascoric Acid 10-NONADECANONE 3-EICOSANONE DEHYDROCHOLIC ACID ETHYL ESTER 7-OXODODECANOIC ACID 7-OXOOCTANOIC ACID 9-oxodecanoic acid phosphoric acid Glycocholic acid 7-OXONONANOIC ACID) 4-PENTYLBICYCLOHEXYL-4-ONE 5-BETA-CHOLANIC ACID-3,7,12-TRIONE METHYL ESTER trans-4-(trans-4-Butylcyclohexyl)cyclohexylanone 4-Pentyldicyclohexylanone

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