Guanidine thiocyanate

Guanidine thiocyanate Basic information
Description Uses Biochem/physiol Actions Toxicity evaluation
Product Name:Guanidine thiocyanate
Synonyms:GUANIDINE HYDROTHIOCYANATE;GUANIDINE MONOTHIOCYANATE;GUANIDINE RHODANIDE;GUANIDINIUM RHODANIDE;AMINOMETHANAMIDINE THIOCYANATE;Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate;Thiocyanic acid, compound with guanidine (1:1);GdmSCN
CAS:593-84-0
MF:CH5N3.CHNS
MW:118.16
EINECS:209-812-1
Product Categories:proteinmod;Pharmaceutical Intermediates;Sulphur Derivatives;bulk;PP;monomer;593-84-0;BDO
Mol File:593-84-0.mol
Guanidine thiocyanate Structure
Guanidine thiocyanate Chemical Properties
Melting point 120-122 °C(lit.)
density 1.103 g/mL at 20 °C
vapor pressure 0Pa at 20℃
refractive index n20/D 1.482
storage temp. 20-25°C
solubility H2O: 6 M at 20 °C, clear, colorless
form Crystals or Crystalline Powder
pka12.5[at 20 ℃]
Specific Gravity1.152
color White or colorless
PH4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution)
OdorOdorless
PH Range4.8 - 6.0
Water Solubility Soluble in water and ethanol
Sensitive Light Sensitive
BRN 3563461
Stability:Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.
InChIKeyZJYYHGLJYGJLLN-UHFFFAOYSA-N
LogP-1.5--1.42 at 20℃
CAS DataBase Reference593-84-0(CAS DataBase Reference)
EPA Substance Registry SystemThiocyanic acid, compd. with guanidine (1:1) (593-84-0)
Safety Information
Hazard Codes Xn,T,C
Risk Statements 20/21/22-32-52/53-36/37/38-34
Safety Statements 36/37-61-13-36-26-45-36/37/39
RIDADR 1759
WGK Germany 2
RTECS XL1225000
3-10
TSCA Yes
HazardClass 8
PackingGroup III
HS Code 29252000
MSDS Information
ProviderLanguage
Guanidine thiocyanate English
SigmaAldrich English
ACROS English
ALFA English
Guanidine thiocyanate Usage And Synthesis
DescriptionGuanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
UsesGuanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.
Biochem/physiol ActionsGuanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.
Toxicity evaluationGuanidine thiocyanate is used commercially as a disinfectant and a general protein denaturant but its more common use is in the extraction of DNA and RNA in molecular biology. Нere are no reports of guanidine thiocyanate toxicity from oral ingestion. Toxicity of diوٴerent thiocyanate compounds other than guanidine thiocyanate has been described. Potassium thiocyanate was used over 50 years ago for the treatment of hypertension and its toxicity appears to cause a neurological disorder consisting of generalized weakness, delirium and a decrease level of consciousness. Reports of thiocyanate toxicity from nitroprusside use for hypertension in the setting of renal impairment/failure have also been reported as thiocyanate is renally cleared. In a fatal deliberate ingestion of a herbicide which contained ammonium thiocyanate and aminotriazole, a 54 year old man presented with coma and cardiovascular collapse although the relative contributions of the two herbicides to the clinical toxicity is unknown.
Chemical PropertiesWhite cryst.powder
UsesGuanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.
UsesPotent protein denaturant used in isolation of intact DNA, RNA.
UsesGuanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid. It is a chaotropic agent, mainly involved in the extraction of DNA and RNA. It is utilized to deactivate the influenza virus.
General DescriptionGuanidine thiocyanate is chaotropic salts which lyse cells and along with nuclease binding matrix it is also seen to inhibit nuclease activity during extraction of DNA. It works as a strong RNase inhibitor in extraction buffers.
HazardIrritant.
Flammability and ExplosibilityNotclassified
Biochem/physiol ActionsGuanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.
Guanidine thiocyanate Preparation Products And Raw materials
6-FITC RHODAMINE B ISOTHIOCYANATE ETHYL ISOTHIOCYANATOACETATE FLUORESCEIN ISOTHIOCYANATE-DEXTRAN COBALT THIOCYANATE GUANIDINE THIOCYANATE - SOLUTION (4 M IN 0.1 M TRIS Guanidine Thiocyanate 4M Solution GUANIDINE RHODANIDE (GUANIDINE THIOCYANATE) GUANIDINE THIOCYANATE - SOLUTION (6 M IN 0.1 M TRIS GUANIDINE THIOCYANATE SOLUTION BUFFERED thiocyanate { GUANIDINE THIOCYANATE 500 Guanidine thiocyanate Solution, buffered pH 7.5 GUANIDINE THIOCYANATE (BIOTECH GRADES) Guanidine-2-cation GUANIDINE { GUANIDINE THIOCYANATE 1 GUANIDINE THIOCYANATE-D(6)* Guanidine Thiocyanate Solution Guanidinium Thiocyanate

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