DRB

DRB Basic information
Product Name:DRB
Synonyms:5,6-DICHLOROPURINE-1-BETA-D-RIBOFURANOSYL-H-BENZIMIDAZOLE;5,6-DICHLORO-1-BETA-D-RIBOFURANOSYLBENZIMIDAZOLE;5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazol;5,6-dichlorobenzimidazoleriboside(drb);Dichlorobenzimidazoleribofuranoside;dichlororibofuranosylbenzimidazole;5,6-Dichloro-1-b-D-ribofuranosylbenzimidazole;5,6-Dichloropurine-1-b-D-ribofuanosyl-H-benzimidazole
CAS:53-85-0
MF:C12H12Cl2N2O4
MW:319.14
EINECS:
Product Categories:Bases & Related Reagents;Inhibitors;Nucleotides
Mol File:53-85-0.mol
DRB Structure
DRB Chemical Properties
Melting point 222-224°C
Boiling point 606.2±65.0 °C(Predicted)
density 1.5917 (rough estimate)
refractive index 1.6200 (estimate)
storage temp. -20°C
solubility Soluble in DMSO (up to 20 mg/ml)
pka13.23±0.70(Predicted)
form White solid
color White
Water Solubility Soluble in DMSO to 75mMSoluble in dimethyl sulfoxide, hot ethanol, dimethyl formamide, (1:2) dimethyl formamide:Phosphate buffer saline, water, methanol and pyridine. Slightly soluble in aqueous buffers.
λmax296nm(lit.)
BRN 39123
Stability:Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.
InChIKeyXHSQDZXAVJRBMX-AYLPWXGPSA-N
CAS DataBase Reference53-85-0
Safety Information
Safety Statements 24/25
WGK Germany 3
RTECS DD7310000
HS Code 29349990
MSDS Information
ProviderLanguage
SigmaAldrich English
DRB Usage And Synthesis
DescriptionDRB (53-85-0) is a classic inhibitor of transcription by RNA polymerase II.?A relatively selective inhibitor of Cdk9 (IC50=3 μM), the kinase of the positive transcription elongation factor b (P-TEF-b) required for processive transcription elongation by RNA polymerase II.1,2?Also inhibits casein kinase II, IC50=4-10 μM.?3?Suppresses the SIRT1/CK2α pathway and enhances the radiosensitivity of human cancer cells.4?Kinase-independent activities of Cdk9 such as glucocorticoid receptor modulation are not inhibited by DRB.5
Chemical PropertiesWhite Solid
Uses5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside has been used:
  • as an inhibitor of RNA polymerase II in mouse melanoma cells
  • for the inhibition of cyclin D1 mRNA synthesis in human prostate epithelial cell lines
  • in the inhibition of interleukin-2 gene transcription in Jurkat cells

UsesDRB is an inhibitor of RNA synthesis (causes premature termination of transcription).
UsesInhibits casein kinase II and blocks RNA synthesis. An adenosine analog.5,6-Dichlorobenzimidazole riboside is used as an inhibitor of RNA Polymerase II. It is nucleoside analogue and used in research to study the mechanisms of cellular regulation. Further, it inhibits some protein kinases.
Biochem/physiol Actions5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB), a nucleoside analog that halts mRNA synthesis by phosphorylation of the C-terminal domain of RNA polymerase II, making it inactive. It also interferes with the DNA topoisomerase II, may modulate response to cytokines and blocks the human immunodeficiency virus (HIV) via RNA modification. It also inhibits cyclin-dependent kinases (CDKs) 7 and 9 and favors apoptosis in leukemic cells. It may serve as a therapeutic agent in treating cancer.
References1) Baumli?et al.?(2010),?Halogen bonds form the basis for selective P-TEFb inhibition by DRB; Chem. Biol.,?17?931 2) Yamaguchi?et al.?(1998),?Interplay between positive and negative elongation factors: drawing a new view of DRB; Genes Cells,?3?9 3) Zandomeni (1989), Kinetics of inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole; Biochem.J., 262 469 4) Wang?et al.?(2014),?Inhibition of P-TEFb by DRB suppresses SIRT1/CK2α pathway and enhances radiosensitivity of human cancer cells; Anticancer Res.,?34?6981 5) Zhu?et al.?(2014),?A kinase-independent activity of Cdk9 modulates glucocorticoid receptor-mediated gene induction; Biochemistry,?53?1753
DRB Preparation Products And Raw materials
HLA-DRB4 5,6-DICHLOROBENZIMIDAZOLE DRB 5,6-DICHLORO-1-BETA-D-RIBOFURANOSYLBENZIMIDAZOLE-3',5'-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT N-Ethylbenzimidazole N-ETHYL-5,6-DICHLOROBENZIMIDAZOLE 1-HYDROXYMETHYLBENZIMIDAZOLE 1H-Benzimidazole,1-propyl-(9CI) BoLA-DRB3 antigen (S)-3-AMINO-1,2-DIHYDROXYPROPANE HYDROCHLORIDE HLA-DRB5 HLA-DRB1 6-CHLORO-1H-BENZIMIDAZOLE HLA DRB4*01033 SP-5,6-DCI-CBIMPS HLA-DRB8 HLA-DRB6 BASIONIC(TM) AC 75

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